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CURRICULUM VITAE
James G. Tokuhisa
Department of
Horticulture
Latham Hall Rm 503
Blacksburg, VA 24061
(540) 231-5653 FAX 231-3347
E-mail=tokuhisa@vt.edu
EDUCATION
Ph.D., Botany, minor in Biochemistry, 1986, University of Wisconsin,
Madison
B.S., Botany with Distinction, 1979, University of Illinois, Urbana
EMPLOYMENT
2005-pres Assistant Professor of Horticulture, Virginia Tech
2000-2005 Research Scientist, Max-Planck-Institute for Chemical
Ecology
1999-2000 Visiting Scientist, Max-Planck-Institute for Chemical
Ecology
1997-2000 Assistant Scientist, Washington State University
1995-1996 Lab Coordinator, Washington State University
1991-1996 Research Associate, Washington State University
1986-1990 Research Scientist, Division of Plant
Industry, CSIRO, Australia
1986 (Nov) Visiting Scientist, National Inst. Basic Biology,
Okazaki, Japan
1981-1986 Research Assistant, University of Wisconsin-Madison
PEER-REVIEWED PUBLICATIONS (21)
Uddin, M. M., Ulrichs, C., Tokuhisa, J. G., and Mewis, I. (2009) Impact of glucosinolate structure on the performance of the crucifer pest Phaedon cochleariae (F.). J. Appl. Bot. Food Qual. 82(2), 108-113. (Cited: 1, ISI 2010)
Ginzberg, I., Tokuhisa, J.G., and Veilleux, R.E. (2009) Potato
steroidal glycoalkaloids: biosynthesis and genetic manipulation.
Potato Res. 52 (1), 1-15.
Falk, K.L., Tokuhisa, J.G., and Gershenzon, J. (2007) The effect of
sulfur nutrition on plant glucosinolate content: physiology and
molecular mechanisms. Plant Biol., 9 (5), 573-581. (cited: 14, ISI
2010)
Textor, S., de Kraker, J.-W., Hause, B., Gershenzon, J., Tokuhisa,
J.G. (2007) MAM3 catalyzes the formation of all aliphatic
glucosinolate chain lengths in Arabidopsis thaliana. Plant
Physiol. 144 (1), 60-71. (cited: 26, ISI 2010)
de Kraker, J-W., Luck, K., Textor, S., Tokuhisa, J.G., Gershenzon,
J. (2007) Two Arabidopsis genes (IPMS1 and IPMS2)
encode isopropylmalate synthase, the branchpoint step in the
biosynthesis of leucine. Plant Physiol. 143, 970-986. (Cited: 14, ISI 2010)
Mewis, I., Tokuhisa, J.G., Schultz, J.C., Appel, H.M., Ulrichs, C.,
and Gershenzon, J. (2006). Gene expression and glucosinolate
accumulation in Arabidopsis thaliana in response to
generalist and specialist herbivores of different feeding guilds and
the role of defense signaling pathways. Phytochemistry 67,
2450-2462. (cited: 47, ISI 2010)
Hirai, M.Y., Klein, M., Fujikawa, Y., Yano, M., Goodenowe, D.B.,
Yamazaki, Y., Kanaya, S., Nakamura, Y., Kitayama, M., Suzuki, H.,
Sakurai, N., Shibata, D., Tokuhisa, J., Reichelt, M., Gershenzon,
J., Papenbrock, J., and Saito, K. (2005) Elucidation of Gene-to-Gene
and Metabolite-to-Gene Networks in Arabidopsis by Integration of
Metabolomics and Transcriptomics. J. Biol. Chem. 280, 25590-25595.
(cited 127, ISI 2010)
Jost, R., Altschmied, L., Bloem, E., Bogs, J., Gershenzon, J.,
Haehnel, U., Haensch, R., Hartmann, T., Kopriva, S., Kruse, C.,
Mendel, RR., Papenbrock, J., Reichelt, M., Rennenberg, H., Schnug,
E., Schmidt, A., Textor, S., Tokuhisa, J., Wachter, A., Wirtz, M.,
Rausch, T., and Hell, R. (2005) Expression profiling of metabolic
genes in response to methyl jasmonate reveals regulation of genes of
primary and secondary sulfur-related pathways in Arabidopsis
thaliana. Photosynthesis Research 86, 491-508. (cited 21, ISI
2010)
Brown, P.D., Tokuhisa, J. G., Reichelt, M.,
Gershenzon, J. (2003) Variation of glucosinolate accumulation among
different organs and developmental stages of Arabidopsis thaliana.
Phytochemistry 62, 471-481. (cited 140, ISI 2010)
Reichelt, M., Brown, P.D., Schneider, B., Oldham, N.J., Stauber, E.,
Tokuhisa, J., Kliebenstein, D.J., Mitchell-Olds, T., Gershenzon, J.
(2002) Benzoic acid glucosinolate esters and other glucosinolates
from Arabidopsis thaliana. Phytochemistry 59, 663-671.
(cited 78, ISI 2010)
Kroymann, J., Textor, S., Tokuhisa, J., Falk, K., Bartram, S.,
Gershenzon, J., and Mitchell-Olds, T. (2001) A gene controlling
variation in Arabidopsis glucosinolate composition is part of the
methionine chain elongation pathway. Plant Physiol. 127,
1077-10881. (cited 97, ISI 2010)
Tokuhisa, J.G., Vijayan, P.,
Feldmann, K.A. and Browse, J.A. (1998) Chloroplast development at
low temperatures requires a homolog of DIM1, a yeast gene encoding
the 18S ribosomal RNA dimethylase. Plant Cell 10, 699-712. (cited
20, ISI 2010)
Tokuhisa, J.G., LaBrie, S.T., Feldmann, K.A. and Browse, J. (1997)
Mutational analysis of chilling tolerance. Plant, Cell and
Environment 20, 1391-1400. (cited 3,ISI 2010)
Ellis, J.G., Tokuhisa, J.G., Llewellyn, D.J., Bouchez, D., Singh,
K., Dennis, E.S., Peacock, W.J. (1993). Does the ocs-element occur
as a functional component of the promoters of plant genes? Plant J.
4, 433-443. (cited 48,ISI 2010)
Singh, K., Dennis, E.S., Ellis, J.G., Llewellyn, D.J., Tokuhisa,
J.G., Wahleithner, J.A., and Peacock, W.J. (1990). OCSBF1, a maize
Ocs enhancer binding factor: Isolation and expression during
development. Plant Cell 2, 891-903. (cited 109, ISI 2010)
Tokuhisa, J.G., Singh, K., Dennis, E.S., and Peacock, W.J. (1990). A
DNA-binding protein factor recognizes two binding domains within the
octopine synthase enhancer element. Plant Cell 2, 215-224. (cited
46, ISI 2010)
Bouchez, D., Tokuhisa, J.G., Llewellyn, D.J., Dennis, E.S., and
Ellis, J.G. (1989). The ocs-element is a component of the promoters
of several T-DNA and plant viral genes. EMBO J. 8, 4197-4204.
(cited 123, ISI 2010)
Tokuhisa, J.G., and Quail, P.H. (1989). Phytochrome in green
tissue: Partial purification and characterization of the
118-kilodalton phytochrome species from light-grown Avena sativa
L. Photochem. Photobiol. 50, 143-152. (cited 28, ISI 2010)
Singh, K., Tokuhisa, J.G., Dennis, E.S., and Peacock, W.J. (1989).
Saturation mutagenesis of the octopine synthase enhancer:
Correlation of mutant phenotypes with binding of a nuclear protein
factor. Proc. Natl. Acad. Sci. USA 86, 3733-3737. (cited 49, ISI
2010)
Tokuhisa, J.G., and Quail, P.H. (1987). The levels of two distinct
species of phytochrome are regulated differently during germination
in Avena sativa L. Planta 172, 371-377. (cited 34, ISI 2010)
Tokuhisa, J.G., Daniels, S.M., and Quail, P.H. (1985). Phytochrome
in green tissue: Spectral and immunochemical evidence for two
distinct molecular species of phytochrome in light-grown Avena
sativa L. Planta 164, 321-332. (cited 146, ISI 2010)
BOOK CHAPTERS (6)
Tokuhisa, J., de Kraker, J.W., Textor, S., and Gershenzon, J. (2004)
The biochemical and molecular origins of aliphatic glucosinolate
diversity in Arabidopsis thaliana. In: "Recent Advances in
Phytochemistry", Romeo, J.T., ed. (New York: Pergamon).
Tokuhisa, J., and Browse, J. (2000) Genetic engineering of plant
chilling tolerance. In "Genetic Engineering", Setlow, J.K., ed. (New
York: Plenum Press), Vol 21. pp. 79-93.
Tokuhisa, J., Wu, J., Miquel, M., Xin, Z., and Browse, J. (1998).
Investigating the role of lipid metabolism in chilling and freezing
tolerance. In "Plant Cold Hardiness", Li, P. and Chen, T., eds. (New
York: Plenum Press), pp. 153-169.
Dennis, E.S., Walker, J.C., Llewellyn, D.J., Ellis, J.G., Singh, K.,
Tokuhisa, J.G., Wolstenholme, D.R., and Peacock, W.J. (1989). The
response to anaerobic stress: Transcriptional regulation of genes
for anaerobically induced proteins. In "Environmental Stress in
Plants", NATO ASI Series G19, Cherry, J.H., ed. (Heidelberg:
Springer-Verlag), pp. 231- 245.
Dennis, E.S., Walker, J.C., Llewellyn, D.J., Ellis, J.G., Singh, K.,
Tokuhisa, J.G., Wolstenholme, D.R., and Peacock, W.J. (1987).
The response to anaerobic stress: Transcriptional regulation of
genes for anaerobically induced proteins. In "Plant Molecular
Biology", von Wettstein, D. and Chua, N.-H., eds. (New York: Plenum
Press), pp. 407-417.
Vierstra, R.D., Tokuhisa, J.G., and Quail, P.H. (1983). A
solid-phase antibody approach to the subcellular localization of
phytochrome. In "Current Topics in Plant Biochemistry and
Physiology", Randall, D.D., Blevins, D.G., and Larson, R. eds.
(Columbus: University of Missouri), pp. 37-50.
TEACHING AND STUDENT TRAINING
Supervisor Ph.D. granted (Alice Mweetwa, 2009)
Supervisor M.S. Student (Will Wadlington, 2009)
Advisor 2007 Jung-hyun Huh, Sarah Holt
Advisor 2005 Natasha Safaee, Emily
Grant,
Instructor Hort 2234 (2009, '10), Hort4004 Seminar (2006,
'07), BIOL5064
Seminar (2006, '07)
Supervisor Intern Student (Alana McAllister), 2004
Supervisor Diploma Student (Nadine Gerth), 2003-2004
Organizer Journal Club (Institute of Biological Chemistry),
1993 - 1996
Supervisor NSF-REU Fellowship recipient (Dionne Ford), 1995
Supervisor Masters of Science Intern (Lonneke van der Geest),
1988
Organizer Teaching Assistant Workshop in Biology, 1984-1985
Member Biology Tutorial Advisory Committee, 1983-1986
Member Teaching Asst Policies & Procedures
Committee, 1982-84
Coordinator Teaching Assistant Orientation Workshop, 1983
Member Graduate Admissions, Asstships & Fellowships, 1981-83
Teaching Asst. Introductory Botany for Dr. R.F. Evert, 1982
Teaching Asst. Cell Biology Laboratory for Dr. W.M. Becker, 1980
NATIONAL/INTERNATIONAL SYMPOSIUM
ADDRESSES
Phytochemical Society of North America Conference on Biologically Active Phytochemicals, Towson (MD), USA August 2009. “The steroidal glycoalkaloids of Solanum chacoense.”
First International Symposium on Glucosinolates, Jena, Germany
September 2006. "Fressen oder Essen: Glucosinolates and herbivore
feeding on Arabidopsis mutants."
Phytochemical Society of North America Conference on Secondary
Metabolism in Model Systems, Peoria (IL), USA; August 2003.
"Aliphatic glucosinolate biosynthesis in Arabidopsis thaliana:
The evolution of an elongating story."
2nd Symposium on Complex Biological Systems, Kunming,
PRC, May 2002. "The Glucosinolate Triangle: Interactions of Plant
Biosynthesis, Toxicity and Herbivore Interactions."
9th International Conference on Arabidopsis Research, Madison, (WI),
USA; June 1998. "Mutational Analysis of Chilling Resistance."
Applications of Molecular Biology to Agriculture in the Year 2000,
Kagoshima, Japan; November 1995. "Acclimation of Plants to Low
Temperature."
American Society of Plant Physiologists, Portland (OR), USA; August
1994. "Plant Growth at Low Temperatures."
UCLA Symposium on Plant Gene Transfer, Park City (UT), USA; April
1989. "DNA-protein interactions of a general plant transcription
factor - the octopine synthase enhancer element."
Boden Research Conference on the Expression of Integrated Foreign
Genes in Plant and Animal Cells, Thredbo (NSW), Australia; Feb
1989. "DNA-protein interactions of a transcriptional regulatory
element functional in plants."
34th Conference of the New Zealand Genetical Society, Canterbury,
New Zealand; Aug 1988. "Transcriptional regulatory elements of
plant genes."
American Society of Plant Physiologists, Davis (CA), USA; August
1984. "Two distinct molecular species of phytochrome in light-grown
Avena sativa."
INVITED SEMINARS (17)
Institute for Advanced Learning, Danville (Mar 2007), Virginia Tech
(Feb 2009, Oct 2005, Feb 2004), Institute of Plant Genetics and Crop Plant
Research, Gatersleben (Jun 2003), M-P-I for Chemical Ecology, Jena
(Jun 2003), Institute for Plant Physiology, Goettingen, (Oct 2000),
Washington State University (Feb 1998, Sep 1994, Feb 1991), Whitman
College (Oct 1994), Purdue University (Oct 1990), USDA-Plant Gene
Expression Ctr (Apr 1989), CSIRO Division of Plant Industry (Nov
1995, Feb 1987), National Institute for Basic Biology (Oct 1986),
University of Wisconsin (Oct 1986).
HONORS AND AWARDS
McKnight Postdoctoral Fellowship, Washington State University, 1991
- 1992
Advanced Opportunity Fellowships, University of Wisconsin, 1979 -
1984
Davis Fund Travel Fellowship, University of Wisconsin, 1983
OTHER PROFESSIONAL EXPERIENCE
Reviewed manuscripts for Journal of Integrative Plant Biology, Phytochemistry, Plant and Cell Physiology,
Plant Cell, Plant Journal, Plant Physiology, Plant Molecular Biology, Plant
Science
Reviewer of grant proposals for the United States Department of
Agriculture (1995-98, 2002, 2005, 2006), National Science Foundation
(2006, 2007, 2009), University of West Virginia (2006), USDA Small Business Innovation Research Program (2006), Binational Agricultural and Research Development Fund
(2006) and the Australian Research Council (1987)
Member of Phytochemical Society of North America (2001) and American
Society of Plant Biologists (1984), Virginia Academy of Science
(2006), and Sigma Xi (2007-present)
EXTERNAL SUPPORT
CALS-Internal Competitive Grant: 2010-2011, Grafted Tomato for Sustainable Field Production, $30,525
Jeffress
Memorial Trust (J-871):
2007-2008 Potato tuber toxins: The biosynthesis of
steroidal glycoalkaloids in Solanum chacoense $22,000
Deutsche Forschungs Gemeinschaft:
2002-2004 The
Function of Glucosinolates and Other Plant Sulfur Compounds in the
Resistance Against Microbial Pathogens (J. Gershenzon, PI)
USDA (97-35100-4200):
1997-2000
Genetic Mechanisms of Chilling Tolerance $110,700
USDA (94-37100-0790): 1994-1996 Genetics of Low Temperature Survival (J.A.
Browse, co-PI) $100,000
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